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Scritto da Annals of Clinical Microbiology and Antimicrobials
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 Background:Four medicinal plants (Chrozophora hierosolymitana Spreng, Chrysanthemum leucanthemumL., Ephedra gerardiana Wall. ex Stapf, and Quercus dilatata L.) used by indigenous healersto treat various infectious diseases were selected for the present study. The major objective ofthe present study was isolation and characterization of antimicrobial components from thecrude plant extracts using bioassay guided fractionation.Methods:Seven methanolic extracts of the four plants were screened to identify any antimicrobialagents present in them. The active crude plant extract was fractionated first by solventpartitioning and then by HPLC. Characterization of the active fractions was done by usingspectrophotometer.Results:All the seven methanolic extracts showed low antifungal activity, however, when theseextracts were tested for antibacterial activity, significant activity was exhibited by twoextracts. The extract of aerial parts of Q. dilatata was most active and therefore, was selectedfor further analysis. Initially fractionation was done by solvent-solvent partitioning and out ofsix partitioned fractions, ethanol fraction was selected on the basis of results of antibacterialactivity and phytochemical analysis. Further, fractionation was carried out by RP- HPLC andpurified active subfractions were characterized by comparing their absorption spectra withthat of the known natural products isolated from the plants of Quercus genus.Discussion and conclusionThe results suggest that this is the first report of the isolated antibacterial compounds fromthis genus.
Fonte: Annals of Clinical Microbiology and Antimicrobials |
